Anti-MBNL3 Antibody Picoband™

Product Name

Anti-MBNL3 Antibody Picoband™

View all MBNL3 Antibodies

SKU/Catalog Number

A08298

Size

100 µg/vial

Form

Lyophilized

Description

Boster Bio Anti-MBNL3 Antibody Picoband™ catalog # A08298. Tested in WB, ICC/IF, ELISA applications. This antibody reacts with Human.

Storage & Handling

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Cite This Product

Anti-MBNL3 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A08298)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Clonality

Polyclonal

Immunogen

E.coli-derived human MBNL3 recombinant protein (Position: L106-C312).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A08298 is reactive to MBNL3 in Human

Applications

A08298 is guaranteed for ELISA, IF, ICC, WB Boster Guarantee

Observed Molecular Weight

27 kDa

Calculated molecular weight

38.532kDa

Background of MBNL3

Muscleblind-like protein 3 is a protein that in humans is encoded by the MBNL3 gene. This gene encodes a member of the muscleblind-like family of proteins. The encoded protein may function in regulation of alternative splicing and may play a role in the pathophysiology of myotonic dystrophy. Alternatively spliced transcript variants have been described.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5 µg/ml, Human
Immunocytochemistry/Immunofluorescence, 5 µg/ml, Human
ELISA, 0.1-0.5 µg/ml, Human

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of MBNL3 using anti-MBNL3 antibody (A08298).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human A431 whole cell lysates,
Lane 2: human HepG2 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MBNL3 antigen affinity purified polyclonal antibody (Catalog # A08298) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MBNL3 at approximately 27 kDa. The expected band size for MBNL3 is at 39 kDa.

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Figure 2. IF analysis of MBNL3 using anti-MBNL3 antibody (A08298) and anti-Beta Tubulin antibody (M01857-3).
MBNL3 was detected in immunocytochemical section of HELA cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-MBNL3 Antibody (A08298) and mouse anti-Beta Tubulin antibody (M01857-3) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) and DyLight®550 Conjugated Goat Anti-Mouse IgG (BA1133) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.

Gene/Protein Information For MBNL3 (Source: Uniprot.org, NCBI)

Alternative Names

CHCRMBLX39FLJ11316; Cys3His CCG1-required protein; FLJ97142; MBXLMBLX; muscleblind-like 3 (Drosophila); muscleblind-like protein 3; Muscleblind-like X-linked protein; Protein HCHCR MBNL3 CHCR, MBLX, MBLX39, MBXL muscleblind like splicing regulator 3 muscleblind-like protein 3|Cys3His CCG1-required protein|muscleblind-like 3|muscleblind-like X-linked protein

For more info on MBNL3, check out the MBNL3 Infographic

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In this infographic, you will see the following information for MBNL3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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